Difference between revisions of "Part:BBa K2429124"

Latest revision as of 19:50, 13 October 2017

Nuclear Localization Sequence (beginning)

This part codes for a peptide sequence that, when placed upstream of a gene sequence, codes for a peptide that indicates that the remaining portion of the translated protein will remain in the cell (specifically in the nucleus) rather then get excreted by the cell. The translated amino acid sequence of the NLS includes PKKKRRV and is classified as a classic nuclear localization sequence (cNLS). Such sequences are considered the best characterized transport signal [1]. This particular sequence exists immediately before the start codon of a coding sequence, and includes a glycine-serine linker (GS) right after the cNLS to link the coding sequence to the NLS.

[1] Lange, et al. "Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α*"

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 22
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 19:32, 13 October 2017 (view source) Nmyrie (Talk \| contribs) ← Older edit		Latest revision as of 19:50, 13 October 2017 (view source) Nmyrie (Talk \| contribs)
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	<partinfo>BBa_K2429124 short</partinfo>		<partinfo>BBa_K2429124 short</partinfo>

−	This part codes for a peptide sequence that, when placed upstream of a gene sequence, codes for a peptide that indicates that the remaining portion of the translated protein will remain in the cell (specifically in the nucleus) rather then get excreted by the cell. The translated amino acid sequence of the NLS includes PKKKRRV and is classified as a classic nuclear localization sequence (cNLS). Such sequences are considered the best characterized transport signal [1].	+	This part codes for a peptide sequence that, when placed upstream of a gene sequence, codes for a peptide that indicates that the remaining portion of the translated protein will remain in the cell (specifically in the nucleus) rather then get excreted by the cell. The translated amino acid sequence of the NLS includes PKKKRRV and is classified as a classic nuclear localization sequence (cNLS). Such sequences are considered the best characterized transport signal [1]. This particular sequence exists immediately before the start codon of a coding sequence, and includes a glycine-serine linker (GS) right after the cNLS to link the coding sequence to the NLS.