Difference between revisions of "Part:BBa K2361009:Design"

 
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===References===
 
===References===
 +
de Ruyter PG, Kuipers OP, Beerthuyzen MM, van Alen-Boerrigter I, de Vos WM. J Bacteriol. 1996 Jun;178(12):3434-9
 +
 +
Kuipers OP, de Ruyter PG, Kleerebezem M, de Vos WM. Journal of Biotechnology. 1998;64:15–21

Latest revision as of 10:12, 27 October 2017


Nisin inducable lactococcal promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This strong promoter can potentially be used to express the different CRISPR parts of the detection system.


Source

The promoter sequence was amplified from a pNZ8048 plasmid obtained from the Department of Molecular Genetics, Nijenborgh 7, 9747 AG, the Netherlands.

References

de Ruyter PG, Kuipers OP, Beerthuyzen MM, van Alen-Boerrigter I, de Vos WM. J Bacteriol. 1996 Jun;178(12):3434-9

Kuipers OP, de Ruyter PG, Kleerebezem M, de Vos WM. Journal of Biotechnology. 1998;64:15–21