Difference between revisions of "Part:BBa K2246006"
 
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siRNA sequence for silencing protein green flourescent protein (GFP) gene.
 
siRNA sequence for silencing protein green flourescent protein (GFP) gene.
 
This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for green flourescent protein (GFP) gene. Designed to be used as negative control during RNA interference experiments.  In previous studies involving RNA interferance in D.citri, a dsRNA for GFP was used as negative control, see Taning, Andrade, Hunter, Christiaens & Smagghe (2016), El-Shesheny, Hajeri, El-Hawary, Gowda & Killiny (2013) and Yu, Gowda & Killiny (2017).
 
This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for green flourescent protein (GFP) gene. Designed to be used as negative control during RNA interference experiments.  In previous studies involving RNA interferance in D.citri, a dsRNA for GFP was used as negative control, see Taning, Andrade, Hunter, Christiaens & Smagghe (2016), El-Shesheny, Hajeri, El-Hawary, Gowda & Killiny (2013) and Yu, Gowda & Killiny (2017).
 +
El-Shesheny I, Hajeri S, El-Hawary I, Gowda S, Killiny N .(2013). Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality.  PLoS ONE 8(5): e65392. doi:10.1371/journal.pone.0065392                                                                                                                                    Taning C., Andrade E., Hunter W., Christiaens O. & Smagghe G. (2016). Asian Citrus Psyllid RNAi Pathway –RNAi evidence. Scientific Reports.  DOI: 10.1038/srep38082                                    Yu X., Gowda S. & Killiny N. (2017). Double-stranded RNA delivery throughsoaking mediates silencing of the muscleprotein 20 and increases mortality to the Asiancitrus psyllid, Diaphorina citri. Pest Management Science. DOI 10.1002/ps.4549
  
  

Latest revision as of 18:46, 12 October 2017


anti-GFP siRNA

siRNA sequence for silencing protein green flourescent protein (GFP) gene. This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for green flourescent protein (GFP) gene. Designed to be used as negative control during RNA interference experiments. In previous studies involving RNA interferance in D.citri, a dsRNA for GFP was used as negative control, see Taning, Andrade, Hunter, Christiaens & Smagghe (2016), El-Shesheny, Hajeri, El-Hawary, Gowda & Killiny (2013) and Yu, Gowda & Killiny (2017). El-Shesheny I, Hajeri S, El-Hawary I, Gowda S, Killiny N .(2013). Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality. PLoS ONE 8(5): e65392. doi:10.1371/journal.pone.0065392 Taning C., Andrade E., Hunter W., Christiaens O. & Smagghe G. (2016). Asian Citrus Psyllid RNAi Pathway –RNAi evidence. Scientific Reports. DOI: 10.1038/srep38082 Yu X., Gowda S. & Killiny N. (2017). Double-stranded RNA delivery throughsoaking mediates silencing of the muscleprotein 20 and increases mortality to the Asiancitrus psyllid, Diaphorina citri. Pest Management Science. DOI 10.1002/ps.4549


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]