Difference between revisions of "Part:BBa K2404013"

Revision as of 14:15, 27 October 2017

Luc+ gene under control of the 35S CaMV promoter

We generated a luciferase construct to test the efficacy of the tobacco leave expression system.

This construct contains the 35S-OTMV promotor the luciferase coding sequence and the Nos terminator.

This construct was introduced into agrobacteria strain GV3101, infiltrated into leaves of Nicotiana benthamiana and the luciferase expression was assessed following addition of luciferin. This full experimental details and results are outlined in the Design and Experience pages.

This part is contained within the Phytobrick level 1 pGB-A1 plasmid.

Our luc+ reporter gene under control of the 35S constitutive promoter from the cauliflower mosaic virus. Luciferase reporter assays were carried out using this construct, with the aim to compare this reading to that of other constructs. This part has the NosT terminator from the Nopaline synthase gene, native to Agrobacterium Tumefaciens . This part should be used as a reporter gene to test 35S expression, relative to another promoter/gene construct.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 13
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 13
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 13
Illegal BglII site found at 400
Illegal BglII site found at 1146
Illegal BglII site found at 2270
Illegal BamHI site found at 2116
Illegal XhoI site found at 1350
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 13
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 13
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1442

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2404013 short</partinfo>
-Our luc+ reporter gene under control of the 35S constitutive promoter from the cauliflower mosaic virus. Luciferase reporter assays were carried out using this construct, with the aim to compare this reading to that of other constructs. This part has the NosT terminator from the Nopaline synthase gene, native to <i>Agrobacterium Tumefaciens </i>. This part is contained within the pGB-A2 plasmid. This part should be used as a reporter gene to test 35S expression, relative to another promoter/gene construct.
+We generated a luciferase construct to test the efficacy of the tobacco leave expression system.
+This construct contains the [https://parts.igem.org/Part:BBa_P10003 35S-OTMV promotor] the luciferase coding sequence and the [https://parts.igem.org/Part:BBa_P10401 Nos terminator].
+This construct was introduced into agrobacteria strain GV3101, infiltrated into leaves of [https://en.wikipedia.org/wiki/Nicotiana_benthamiana Nicotiana benthamiana] and the luciferase expression was assessed following addition of luciferin. This full experimental details and results are outlined in the Design and Experience pages.
+This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10501 pGB-A1 plasmid].
+Our luc+ reporter gene under control of the 35S constitutive promoter from the cauliflower mosaic virus. Luciferase reporter assays were carried out using this construct, with the aim to compare this reading to that of other constructs. This part has the NosT terminator from the Nopaline synthase gene, native to <i>Agrobacterium Tumefaciens </i>.  This part should be used as a reporter gene to test 35S expression, relative to another promoter/gene construct.
 <!-- Add more about the biology of this part here