Difference between revisions of "Part:BBa K2242384"

 
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__NOTOC__
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<partinfo>BBa_K2242384 short</partinfo>
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KMADH is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature.
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To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control it’s expression, which is induced by IPTG.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2242384 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2242384 parameters</partinfo>
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Revision as of 11:40, 12 October 2017


placI+lacI+pTAC+KmAdh

KMADH is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature. To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control it’s expression, which is induced by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1353
    Illegal BglII site found at 3620
    Illegal XhoI site found at 3682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]