Difference between revisions of "Part:BBa K2242419"

 
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<partinfo>BBa_K2242419 short</partinfo>
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As we have mentioned, it’s the heme attached to the Mtr A and Mtr C that enable them to shuttle electrons. So these two protein belong to a protein family called cytochrome c. For those proteins in this family, they all need to get through a process before they become a mature one, which means they can function as they are meant to, the covalent heme ligation. First, those apo-cytochrome c will be secreted to the periplasm, then a protein complex——ccm A-H will catalyze the process of covalent heme ligation, to attach the heme onto the apo-cytochrom c at the right position, after which our cytochrome c proteins can become mature and function as we expected. In our project, we co-express this ccm A-H protein complex with cytochrome c Mtr CAB to mature Mtr CAB to shuttle electrons from electrode into cytoplasm of our engineered E.coli to increase the synthesis efficiency.
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We use a constitutively on promoter pTET to control gene ccmA-H’s expression.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2242419 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2242419 parameters</partinfo>
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Revision as of 11:04, 12 October 2017


pTET+ccmA-H

As we have mentioned, it’s the heme attached to the Mtr A and Mtr C that enable them to shuttle electrons. So these two protein belong to a protein family called cytochrome c. For those proteins in this family, they all need to get through a process before they become a mature one, which means they can function as they are meant to, the covalent heme ligation. First, those apo-cytochrome c will be secreted to the periplasm, then a protein complex——ccm A-H will catalyze the process of covalent heme ligation, to attach the heme onto the apo-cytochrom c at the right position, after which our cytochrome c proteins can become mature and function as we expected. In our project, we co-express this ccm A-H protein complex with cytochrome c Mtr CAB to mature Mtr CAB to shuttle electrons from electrode into cytoplasm of our engineered E.coli to increase the synthesis efficiency. We use a constitutively on promoter pTET to control gene ccmA-H’s expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 534
    Illegal BamHI site found at 3323
    Illegal BamHI site found at 3551
    Illegal BamHI site found at 5895
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 188
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4602