Difference between revisions of "Part:BBa K2229400"

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A Proteorhodopsin-based construct that employs the strong promoter/strong RBS (K880005) combination to up-regulate expression of Proteorhodopsin, a membrane protein with two lysine residue sites (57 and 59) used to bind citrate through electrostatic interactions.
 
A Proteorhodopsin-based construct that employs the strong promoter/strong RBS (K880005) combination to up-regulate expression of Proteorhodopsin, a membrane protein with two lysine residue sites (57 and 59) used to bind citrate through electrostatic interactions.
 
 
  
 
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===Usage and Biology===
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===Characterization===
  
 
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Revision as of 08:59, 25 October 2017


Proteorhodopsin-Expressing Construct

A Proteorhodopsin-based construct that employs the strong promoter/strong RBS (K880005) combination to up-regulate expression of Proteorhodopsin, a membrane protein with two lysine residue sites (57 and 59) used to bind citrate through electrostatic interactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]