Difference between revisions of "Part:BBa K2235009:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | His tag is N-terminally attached to the | + | His tag is N-terminally attached to the Sialidase enzyme coding sequence. |
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===Source=== | ===Source=== |
Revision as of 17:26, 25 October 2017
Sialidase composite with T7 promoter and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 127
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 649
Illegal NgoMIV site found at 739 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1119
Design Notes
His tag is N-terminally attached to the Sialidase enzyme coding sequence.
Source
http://onlinelibrary.wiley.com/doi/10.1042/BA20040144/abstract
Artherobacter Urefaciens