Difference between revisions of "Part:BBa K2206008:Design"
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No terminators are needed as the pSB1C3 plasmid contains terminators. | No terminators are needed as the pSB1C3 plasmid contains terminators. | ||
| − | A non-leaky inducible promoter is needed to amplify the constructs in E.coli as | + | A non-leaky inducible promoter is needed to amplify the constructs in E.coli as low concentrations of the transcript is most likely toxic to E.coli. |
There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein. | There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein. | ||
Revision as of 23:26, 30 October 2017
Toehold switch for hsa-miR-15b-5p with luciferase and inducible promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 2102
Design Notes
No terminators are needed as the pSB1C3 plasmid contains terminators.
A non-leaky inducible promoter is needed to amplify the constructs in E.coli as low concentrations of the transcript is most likely toxic to E.coli.
There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein.
Source
This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.