Difference between revisions of "Part:BBa K2255006"
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| − | This enzyme has been created to disrupt the biofilm of ''X. fastidiosa'', see our wiki for more information about [http://2017.igem.org/Team:Aix-Marseille/DEPS our biofilm part] | + | This enzyme has been created to disrupt the biofilm of ''X. fastidiosa'', see our wiki for more information about [http://2017.igem.org/Team:Aix-Marseille/DEPS our biofilm part]. |
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Revision as of 12:17, 6 October 2017
EPS-Depolymerase (Rfc25)
Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids from the phage vB_XveM_DIBBI which contains two functions, one structural and one pectin lyase. Xanthomonas is a close relative of Xylella fastidiosa.
This part was designed with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domains.
Usage and Biology
This enzyme has been created to disrupt the biofilm of X. fastidiosa, see our wiki for more information about [http://2017.igem.org/Team:Aix-Marseille/DEPS our biofilm part].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]