Difference between revisions of "Part:BBa K2404005"

Revision as of 12:01, 6 October 2017

WRKY30 - a promoter induced by the presence of cellulose-derived oligomers

The WRKY30 promotor is a regulatory sequence from Arabidopsis thaliana that has been characterised as a [http://www.plantphysiol.org/content/early/2017/02/27/pp.16.01680 ‘Damage Associated Molecular Patterns’ (DAMP)].

This promotor is situated -653 > -168 upstream of the WRKY30 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the pSB1C3 plasmid using BsmBI.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 540
Illegal PstI site found at 582
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 540
Illegal PstI site found at 582
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 607
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 540
Illegal PstI site found at 582
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 540
Illegal PstI site found at 582
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 794

@@ Line 5: / Line 5: @@
 The [https://apps.araport.org/thalemine/report.do?id=69089924&trail=|69089924 WRKY30 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been characterised as a [http://www.plantphysiol.org/content/early/2017/02/27/pp.16.01680 ‘Damage Associated Molecular Patterns’ (DAMP)].
-This promotor is situated -653 > -168 upstream of the WRK30 translational start point.
+This promotor is situated -653 > -168 upstream of the WRKY30 translational start point.
 This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.