Difference between revisions of "Part:BBa K2447014"
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<partinfo>BBa_K2447014 short</partinfo> | <partinfo>BBa_K2447014 short</partinfo> | ||
− | pLac promoter | + | In the absence of IPTG, lacI repressor will bind to pLac promoter and inhibit further downstream expression. pLac promoter is attached upstream of the tlpa36 protein coding sequence to control the amount of tlpa36 protein expression. When IPTG is present and under high temperatures (above 36 degree), pTlpa36 will be constitutively and eliciting downstream GFP expression. Under low temperatures (below 36 degree), the pTlpa36 will be repressed, and drastically reducing GFP expression |
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Revision as of 23:14, 9 October 2017
IPTG-inducible temperature-sensitive system with GFP reporter
In the absence of IPTG, lacI repressor will bind to pLac promoter and inhibit further downstream expression. pLac promoter is attached upstream of the tlpa36 protein coding sequence to control the amount of tlpa36 protein expression. When IPTG is present and under high temperatures (above 36 degree), pTlpa36 will be constitutively and eliciting downstream GFP expression. Under low temperatures (below 36 degree), the pTlpa36 will be repressed, and drastically reducing GFP expression
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1750
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1147
Illegal XhoI site found at 2035 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2338
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3303