Difference between revisions of "Part:BBa K2443001"

Revision as of 23:10, 31 October 2017

ArgRS optimized for expression in E. coli

Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1805
Illegal SapI.rc site found at 1523

Revision as of 18:00, 5 October 2017 (view source) Graeme.glaister (Talk \| contribs) ← Older edit		Revision as of 23:10, 31 October 2017 (view source) Harlando (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2443001 short</partinfo>		<partinfo>BBa_K2443001 short</partinfo>

−	Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal ~~histidine~~ tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.