Difference between revisions of "Part:BBa K2443002"

 
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<partinfo>BBa_K2443002 short</partinfo>
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Asparagine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2443003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2443003 parameters</partinfo>
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Revision as of 17:12, 5 October 2017


AsnRS optimized for expression in E. coli

Asparagine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752