Difference between revisions of "Part:BBa K2447012"

Revision as of 09:13, 25 October 2017

Coding region for temperature sensitive protein TlpA36

Tlpa36 is a temperature sensitive protein which is capable of binding to promoter pTlpa36. Under temperature of above 36 degree celsius, tlpa36 protein would form de-dimerise to form monomers and hence it loses the ability to bind to the promoter pTlpa36. If the temperature drops below 36 degree celsius, Tlpa36 proteins would form dimers; which are capable of repressing the pTlpa36 promoter, via steric hindrance to the promoter site, and preventing the recruitment of RNA polymerase to elicit downstream gene expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 508
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 793
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1096
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2447012 short</partinfo>
-Tlpa36 is a temperature sensitive protein which is capable of binding to promoter tlpa36. Under temperature of above 36 degree celsius, tlpa36 protein would form dimers and hence it loses the ability to bind to the promoter tlpa36. If the temperature drops below 36 degree celsius, tlpa36 proteins would form de-dimerise to form tlpa36 protein monomers; which are capable of repressing the tlpa36 promoter.
+Tlpa36 is a temperature sensitive protein which is capable of binding to promoter pTlpa36. Under temperature of above 36 degree celsius, tlpa36 protein would form de-dimerise to form monomers and hence it loses the ability to bind to the promoter pTlpa36. If the temperature drops below 36 degree celsius, Tlpa36 proteins would form dimers; which are capable of repressing the pTlpa36 promoter, via steric hindrance to the promoter site, and preventing the recruitment of RNA polymerase to elicit downstream gene expression.
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