Difference between revisions of "Part:BBa K2205001"
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=CFPS Expression of ''J23100-deGFP'' and ''J23100-sfGFP'' Constructs:= Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
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==CFPS Expression of ''J23100-deGFP'' and ''J23100-sfGFP'' Constructs:== Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
  
  

Revision as of 10:08, 2 October 2017


deGFP

deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.


CFPS Expression of deGFP

T--Newcastle--BB CFPS deGFP sfGFP.png


==CFPS Expression of J23100-deGFP and J23100-sfGFP Constructs:== Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.


In vivo Qualitative Comparison

T--Newcastle--BB deGFP.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]