Difference between revisions of "Part:BBa K2368012:Design"

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===Design Notes===
 
===Design Notes===
By the method of PCR,we combine them.
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Firstly, we construct the specific primer that consists of the overlap regions of the T1R2 and YCP. Then, we combine T1R2 with the YCP by overlap extension PCR.
Modifying unnecessary enzyme cutting sites
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===Source===
 
===Source===
  
  
 
===References===
 
===References===

Revision as of 03:10, 14 October 2017


T1R2-YCP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Firstly, we construct the specific primer that consists of the overlap regions of the T1R2 and YCP. Then, we combine T1R2 with the YCP by overlap extension PCR.

Source

References