Difference between revisions of "Part:BBa K2368020"
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<partinfo>BBa_K2368020 short</partinfo>
 
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<p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R3- amilCP (K592010). The part K592009 is blue chromoprotein and can exhibit strong blue color when expressed. Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and C-terminal of amilCP. And hope to show the location of T1R3 through fluorescence microscope for detecting amilCP.</p>
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<p>This part is that sweetness receptor T1R3 combines with the BCP tag. </p>
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<p>The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R3 and BCP tag.</p>
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<p>The result of the part is that the expression of the blue cyanidin protein to detect the expression and the location of the T1R3. </p>
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<!-- Add more about the biology of this part here
 
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Revision as of 03:44, 14 October 2017


Introduction

BCP+T1R3 overlap

This part is that sweetness receptor T1R3 combines with the BCP tag.

The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R3 and BCP tag.

The result of the part is that the expression of the blue cyanidin protein to detect the expression and the location of the T1R3.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]