Difference between revisions of "Part:BBa K2368017"
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<partinfo>BBa_K2368017 short</partinfo>
 
<partinfo>BBa_K2368017 short</partinfo>
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<h3>General</h3>
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Similarly, in order to knock out the gene called Sst2 which have the inhibition to the GpaI who play an important role in the signal transduction, The composite part is composed of the length of 500 bp of homologous arm and the marker of His, and it can replace the gene of Sst2 by homologous recombination, which can eliminate the influence of inhibition of sweetness signal transduction.
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<p>Sst2 protein is an important negative regulatory factor of the pheromone GPSTP. In order to improve the detection sensitivity, we design this part to knock out the sst2 gene.</p>
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<h3>Our experiment</h3>
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<p>Similarly, we design 3 pairs of primers and the marker is Histone synthesis gene, short termed His. We get 3 fragments by PCR and they have the overlap areas with each other. Then, the complete fragment observed by OE-PCR is converted to the yeast. Additionally, the colony is chosen on the relevant nutritional deficiency medium, so that only the positive cloning can survival on it.</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:36, 14 October 2017


Introduction

500 bp of homologous arm＋His

General

Sst2 protein is an important negative regulatory factor of the pheromone GPSTP. In order to improve the detection sensitivity, we design this part to knock out the sst2 gene.

Our experiment

Similarly, we design 3 pairs of primers and the marker is Histone synthesis gene, short termed His. We get 3 fragments by PCR and they have the overlap areas with each other. Then, the complete fragment observed by OE-PCR is converted to the yeast. Additionally, the colony is chosen on the relevant nutritional deficiency medium, so that only the positive cloning can survival on it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1131
    Illegal BglII site found at 1191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1801
  • 1000
    COMPATIBLE WITH RFC[1000]