Difference between revisions of "Part:BBa K2379006"
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<partinfo>BBa_K2379006 short</partinfo>
 
<partinfo>BBa_K2379006 short</partinfo>
  
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The RNA pHmeter with natural scar site(BBa_K2379002) is under the regulation of the pLAC promoter(BBa_R0011). Following the RNA pHmeter, the construct contains GFP (BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). GFP will get expressed only under alkaline conditions
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The RNA pHmeter with natural scar site(BBa_K2379002) is under the regulation of the pLAC promoter(BBa_R0011). Following the RNA pHmeter, the construct contains GFP (BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014).Expression of GFP is regulated by the RNA oHmeter and hence will be produced only at pH 8.4
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:23, 1 October 2017


pH sensitive system with RNA pHmeter (wild type)

The RNA pHmeter with natural scar site(BBa_K2379002) is under the regulation of the pLAC promoter(BBa_R0011). Following the RNA pHmeter, the construct contains GFP (BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014).Expression of GFP is regulated by the RNA oHmeter and hence will be produced only at pH 8.4 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 173
    Illegal BsaI.rc site found at 906