Difference between revisions of "Part:BBa K2379005"

Revision as of 19:15, 22 October 2017

RNA thermometer (FourU) with GFP under the regulation of pLAC

The RNA thermometer (BBa_K115002) is under the regulation of pLAC promoter (BBa_R0011). Following the RNA thermometer, the construct contains GFP(BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). Expression of GFP is regulated by the RNA thermometer and hence will be produced only at 37 degree Celsius.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 765

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 <partinfo>BBa_K2379005 short</partinfo>
-The RNA thermometer (BBa_K115001) is under the regulation of pLAC promoter (BBa_R0011). Following the RNA thermometer, the construct contains GFP(BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). Expression of GFP is regulated by the RNA thermometer and hence will be produced only at 42degree celsius.
+The RNA thermometer (BBa_K115002) is under the regulation of pLAC promoter (BBa_R0011). Following the RNA thermometer, the construct contains GFP(BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). Expression of GFP is regulated by the RNA thermometer and hence will be produced only at 37 degree Celsius.
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