Difference between revisions of "Part:BBa K2239012:Design"

 
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===Design Notes===
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===Source===
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The 7beta-HSDH DNA is artificially synthesized based on the sequence retrieved from the GenBank.
  
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===Source===
 
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The 7beta-HSDH sequence is in the genome of R. torques ATCC 35915, and is ordered from a synthesis company based on its sequence.
 
  
 
===References===
 
===References===
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[1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015.

Revision as of 00:42, 1 November 2017


7beta-HSDH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The 7beta-HSDH DNA is artificially synthesized based on the sequence retrieved from the GenBank.


References

[1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015.