Difference between revisions of "Part:BBa K2450101"

 
Line 3: Line 3:
 
<partinfo>BBa_K2450101 short</partinfo>
 
<partinfo>BBa_K2450101 short</partinfo>
  
−
A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The tag allows for relative quantification of expression.
+
A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:11, 21 October 2017


TEV protease tagged with mCherry

A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1444
  • 1000
    COMPATIBLE WITH RFC[1000]