Difference between revisions of "Part:BBa K2309031"

 
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<partinfo>BBa_K2309031 short</partinfo>
 
<partinfo>BBa_K2309031 short</partinfo>
  
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This primer contain 18 bp pf BioBrick prefix and the rest  for Gibson Assembly. PstI is used to linearize pNZ8148.
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This primer contains 18 bp nucleotides which can anneal standardized biobrick prefix, and a 20-bp-long overhang for Gibson Assembly, which has 20bp overlap with top strand of linearized pNZ8148 digested by PstI.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:03, 28 September 2017


Standard forward primer for Gibson Assembly to plasmid pNZ8148

This primer contains 18 bp nucleotides which can anneal standardized biobrick prefix, and a 20-bp-long overhang for Gibson Assembly, which has 20bp overlap with top strand of linearized pNZ8148 digested by PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
    Illegal NotI site found at 28
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]