Difference between revisions of "Part:BBa K2255005:Design"
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===Design Notes===
 
===Design Notes===
  
−
D3 and the signal sequence are both the best conserved part from the attachment protein. So with protein global alignment (Needleman-Wunsch alignment), from two or three sequence at one time, we were eventually able to determinate D3 from M13.  
+
D3 and the signal sequence are both the best conserved part from the attachment protein. Using a global protein alignment (Needleman-Wunsch alignment), using two or three sequence at one time, we were eventually able to determinate D3 from M13.  
  
 
We used iDT to optimise this sequence for ''E.coli''.
 
We used iDT to optimise this sequence for ''E.coli''.

Revision as of 16:32, 29 September 2017


Domain 3 of p3 from M13 (Rfc25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

D3 and the signal sequence are both the best conserved part from the attachment protein. Using a global protein alignment (Needleman-Wunsch alignment), using two or three sequence at one time, we were eventually able to determinate D3 from M13.

We used iDT to optimise this sequence for E.coli.

Source

The initial sequence came from E.coli M13 filamentous phages. But we modified the sequence with iDT optimisation codon.

References