Difference between revisions of "Part:BBa K2255003"

Revision as of 16:04, 29 September 2017

Multi-Tag (Rfc 25)

This tag provides multiple fixation sites: a streptavidine and a histidine tag. Between those tag is a TEV protease clivage site.

This part was designed with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2255003 short</partinfo>
-This tag provides multiple fixation site: streptavidine and a histidine tag. Between those tag the is a TEV protease clivation site.
+This tag provides multiple fixation sites: a streptavidine and a histidine tag. Between those tag is a TEV protease clivage site.
-This part was design with Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
+This part was designed with the Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain.
 <!-- Add more about the biology of this part here