Difference between revisions of "Part:BBa K2319000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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A HindIII site was created after the RBS to allow the insertion of any protein coding sequence under the T7 expression system by using HindIII and NheI.
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A HindIII site was created after the RBS to allow the insertion of any protein coding sequence under the T7 expression system by using HindIII and NheI. For more detailed information about the design of this part, visit this<a href="http://2017.igem.org/Team:IISc-Bangalore/Design">design page</a>.
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===Source===
 
===Source===

Revision as of 01:21, 2 November 2017


sfGFP-SpyCatcher under T7 expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 768
    Illegal XhoI site found at 1001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 57


Design Notes

A HindIII site was created after the RBS to allow the insertion of any protein coding sequence under the T7 expression system by using HindIII and NheI. For more detailed information about the design of this part, visit this<a href="http://2017.igem.org/Team:IISc-Bangalore/Design">design page</a>.

Source

This part was generated by assembling the T7 promoter, a strong RBS, the sfGFP-SpyCatcher coding sequence, and a T7 terminator. All these parts are available in the Registry.

References