Difference between revisions of "Part:BBa K2273066:Design"
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===Design===
 
===Design===
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===Source===
  
 
The SpoIID signal peptide of <i>B. subtilis</i> lytic transglycosylase was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
 
The SpoIID signal peptide of <i>B. subtilis</i> lytic transglycosylase was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.

Revision as of 18:53, 22 September 2017


SpoIID signal peptide of B. subtilis lytic transglycosylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design

Source

The SpoIID signal peptide of B. subtilis lytic transglycosylase was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

SpoIID_SP fwd gatcGAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGAAACAATTCGCAATCACACTAT
SpoIID_SP rev gatcTCTGCAGCGGCCGCTACTAGTATTAACCGGTGGCCCCCGCTTCCTTATT

Following amplification, the signal peptide was digested using EcoRI and PstI and ligated into pSB1C3.

References