Difference between revisions of "Part:BBa K2273066:Design"
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<partinfo>BBa_K2273066 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2273066 SequenceAndFeatures</partinfo> | ||
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The SpoIID signal peptide of <i>B. subtilis</i> lytic transglycosylase was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below. | The SpoIID signal peptide of <i>B. subtilis</i> lytic transglycosylase was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below. | ||
Revision as of 18:52, 22 September 2017
SpoIID signal peptide of B. subtilis lytic transglycosylase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design
The SpoIID signal peptide of B. subtilis lytic transglycosylase was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.
| SpoIID_SP fwd | gatcGAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGAAACAATTCGCAATCACACTAT |
| SpoIID_SP rev | gatcTCTGCAGCGGCCGCTACTAGTATTAACCGGTGGCCCCCGCTTCCTTATT |
Following amplification, the signal peptide was digested using EcoRI and PstI and ligated into pSB1C3.