Difference between revisions of "Part:BBa K2485012:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | A 5x Gly-Ser linker, BBa_J18922, was used to join proteorhodopsin and cPT. | |
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===Source=== | ===Source=== | ||
Revision as of 19:22, 21 September 2017
Proteorhodopsin-cPT-RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1117
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A 5x Gly-Ser linker, BBa_J18922, was used to join proteorhodopsin and cPT.
Source
The green light absorbing protein rhodopsin was sourced from BBa_K773002 and codons were slightly modified to enhance GC content. It is a proton pump used by marine bacteria for generating proton motive force for ATP generation.
The cPT Region of IcsA was sourced from BBa_K2485011 with removed start and stop codons, and the last four nucleotides were modified to match the fusion sequence between cPT and GFP in a plasmid provided by Marcia B. Goldberg, MD which was experimentally shown to unipolarly localize GFP.
FresnoRFP from Atum was used instead of GFP.