Difference between revisions of "Part:BBa K2485012:Design"

 
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The green light absorbing protein rhodopsin was sourced from BBa_K773002 and codons were slightly modified to enhance GC content. It is a proton pump used by marine bacteria for generating proton motive force for ATP generation.
 
The green light absorbing protein rhodopsin was sourced from BBa_K773002 and codons were slightly modified to enhance GC content. It is a proton pump used by marine bacteria for generating proton motive force for ATP generation.
  
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The cPT Region of IcsA was sourced from BBa_K2485011 with removed start and stop codons, and the last four nucleotides were modified to match the fusion sequence between cPT and GFP in  
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The cPT Region of IcsA was sourced from BBa_K2485011 with removed start and stop codons, and the last four nucleotides were modified to match the fusion sequence between cPT and GFP in a plasmid provided by Marcia B. Goldberg, MD which was experimentally shown to unipolarly localize GFP.
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FresnoRFP from Atum was used instead of GFP.
  
 
===References===
 
===References===

Revision as of 19:14, 21 September 2017


Proteorhodopsin-cPT-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I'll get there!


Source

The green light absorbing protein rhodopsin was sourced from BBa_K773002 and codons were slightly modified to enhance GC content. It is a proton pump used by marine bacteria for generating proton motive force for ATP generation.

The cPT Region of IcsA was sourced from BBa_K2485011 with removed start and stop codons, and the last four nucleotides were modified to match the fusion sequence between cPT and GFP in a plasmid provided by Marcia B. Goldberg, MD which was experimentally shown to unipolarly localize GFP.

FresnoRFP from Atum was used instead of GFP.

References