Difference between revisions of "Part:BBa K2273000:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2273000 short</partinfo>
 
<partinfo>BBa_K2273000 short</partinfo>
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<partinfo>BBa_K2273000 SequenceAndFeatures</partinfo><br>
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<partinfo>BBa_K2273000 SequenceAndFeatures</partinfo>
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===Design===
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This part was generated with RFC10 Suffix and Prefix. It encodes for the <i>srfA</i> promoter that is part of the ComX quorum response of <i>B. subtilis</i> and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br>
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===Design Notes===
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none
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===Source===
 
===Source===
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gDNA of <i>Bacillus subtilis</i>
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The <i>srfA</i> promoter of <i>B. subtilis</i> was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
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<br>
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<table>
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<tr>
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<td width="150">PsrfA fwd RFC10</td>
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<td>gatcGAATTCGCGGCCGCTTCTAGAGTTGCGGCATCCCGCAAAAAATATTG</td>
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</tr>
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<tr>
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<td>PsrfA rev RFC10</td>
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<td>gatcCTGCAGCGGCCGCTACTAGTACTTTATAAGCAGTGAACATG</td>
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</tr>
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</table>
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===References===
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Ulf Brockmeier, Michael Caspers, Roland Freudl, Alexander Jockwer, Thomas Noll and Thorsten Eggert "Systematic Screening of All Signal Peptides from <i>Bacillus subtilis</i>: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria" Journal of Molecular Biology 362 (2006): 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/16930615 PubMed]<br><br>
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Jan Maarten van Dijl and Michael Hecker "<i>Bacillus subtilis</i>: from soil bacterium to super-secreting cell factory" Microbial Cell Factories 12:3 (2013): 1-6. [https://www.ncbi.nlm.nih.gov/pubmed/23311580 PubMed]<br><br>
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Ling lin Fu, Zi Rong Xu, Wei Fen Li, Jiang Bing Shuai, Ping Lu, Chun Xia Hu "Protein secretion pathways in <i>Bacillus subtilis</i>: Implication for optimization heterologous protein secretion" Biotechnology Advances 25 (2007): 1-12. [https://www.ncbi.nlm.nih.gov/pubmed/16997527 PubMed]
  
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===References===
 
 
DOI:10.1016/j.jbiotec.2016.03.002<br>
 
DOI:10.1016/j.jbiotec.2016.03.002<br>
 
PMCID:PMC208261<br>
 
PMCID:PMC208261<br>

Revision as of 14:15, 24 October 2017

PsrfA: a ComA-dependent promoter of Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the srfA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The srfA promoter of B. subtilis was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PsrfA fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAGTTGCGGCATCCCGCAAAAAATATTG
PsrfA rev RFC10 gatcCTGCAGCGGCCGCTACTAGTACTTTATAAGCAGTGAACATG

References

Ulf Brockmeier, Michael Caspers, Roland Freudl, Alexander Jockwer, Thomas Noll and Thorsten Eggert "Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria" Journal of Molecular Biology 362 (2006): 393-402. PubMed

Jan Maarten van Dijl and Michael Hecker "Bacillus subtilis: from soil bacterium to super-secreting cell factory" Microbial Cell Factories 12:3 (2013): 1-6. PubMed

Ling lin Fu, Zi Rong Xu, Wei Fen Li, Jiang Bing Shuai, Ping Lu, Chun Xia Hu "Protein secretion pathways in Bacillus subtilis: Implication for optimization heterologous protein secretion" Biotechnology Advances 25 (2007): 1-12. PubMed

DOI:10.1016/j.jbiotec.2016.03.002
PMCID:PMC208261
DOI: 10.1093/nar/gkv1242
DOI: 10.1099/mic.0.26003-0
DOI: 10.1002/bit.24524