Difference between revisions of "Part:BBa K2215004"

Revision as of 06:07, 1 October 2017

Putative Symbiodinium Promoter

This is putative Symbiodinium promoter that is believed to work best in Symbiodinium due to its unique features, such as a "TTTT-box rather than a TATA box, a unique transcription start site branch point, and acceptor for the dinoflagellate spliced leader" (Levin et al., 2017). With this promoter, it should allow for more expression and stability of transgenes introduced in Symbiodinium.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 6
Illegal suffix found in sequence at 971
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal SpeI site found at 972
Illegal PstI site found at 986
Illegal NotI site found at 12
Illegal NotI site found at 979
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal BglII site found at 360
Illegal BglII site found at 438
Illegal BglII site found at 790
Illegal XhoI site found at 442
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 6
Illegal suffix found in sequence at 972
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 6
Illegal XbaI site found at 21
Illegal SpeI site found at 972
Illegal PstI site found at 986
Illegal AgeI site found at 308
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2215004 short</partinfo>
-This is putative Symbiodinium promoter that is believed to work best in Symbiodinium due to its unique features, such as a "TTTT-box rather than a TATA box, a unique transcription start site branch point, and acceptor for the dinoflagellate spliced leader". With this promoter, it should allow for more expression and stability of transgenes introduced in Symbiodinium.
+This is putative Symbiodinium promoter that is believed to work best in Symbiodinium due to its unique features, such as a "TTTT-box rather than a TATA box, a unique transcription start site branch point, and acceptor for the dinoflagellate spliced leader" (Levin et al., 2017). With this promoter, it should allow for more expression and stability of transgenes introduced in Symbiodinium.
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