Difference between revisions of "Part:BBa K2255009"
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This part is sequence coding for the D1 and D2 of the protein 3 of NgoΦ6, a filamentous phage that target N.gonorrhoeae. Those domains are required for M13 adsorption and entry. | This part is sequence coding for the D1 and D2 of the protein 3 of NgoΦ6, a filamentous phage that target N.gonorrhoeae. Those domains are required for M13 adsorption and entry. | ||
− | This part was design with Freiburg ( | + | This part was design with Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain. |
+ | |||
+ | This part is related to our collection part of filamentous phages attachment protein. To see all the part from this collection, you can check out the first part of this collection : [https://parts.igem.org/Part:BBa_K2255008 BBa_K2255008] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 16:10, 26 September 2017
p3_N.gonorrhoeae (Rfc25)
This part is sequence coding for the D1 and D2 of the protein 3 of NgoΦ6, a filamentous phage that target N.gonorrhoeae. Those domains are required for M13 adsorption and entry.
This part was design with Freiburg (Rfc25) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
This part is related to our collection part of filamentous phages attachment protein. To see all the part from this collection, you can check out the first part of this collection : BBa_K2255008
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]