Difference between revisions of "Part:BBa K2255012"
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This part is sequence coding for the D1 and D2 of the protein 3 of RSS1, a filamentous phage that target R.solanacearum. Those domains are required for phage adsorption and entry.
 
This part is sequence coding for the D1 and D2 of the protein 3 of RSS1, a filamentous phage that target R.solanacearum. Those domains are required for phage adsorption and entry.
  
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This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.  
+
This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:06, 30 August 2017


pIII_R.solanacearum_RSS1 (Rfc25)

This part is sequence coding for the D1 and D2 of the protein 3 of RSS1, a filamentous phage that target R.solanacearum. Those domains are required for phage adsorption and entry.

This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 373
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 172