Difference between revisions of "Part:BBa K2255007"
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The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use ''E. coli'' to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site. | The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use ''E. coli'' to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site. | ||
| − | This part was design with Freiburg (RFC[25]) | + | This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain. |
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Revision as of 13:14, 30 August 2017
Signal sequence of p3 from M13 (Rfc25)
The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use E. coli to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.
This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]