Difference between revisions of "Part:BBa K2255013"

Revision as of 13:02, 30 August 2017

p3_V.Cholerae_CTXΦ (Rfc25)

This part is sequence coding for the D1 and D2 of the protein 3 of CTXΦ, a filamentous phage that target V.Cholerae. Those domains are required for phage adsorption and entry.

This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 438

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2255013 short</partinfo>
-This part is sequence coding for the D1 and D2 of the protein 3 of CTXphi, a filamentous phage that target V.Cholerae. Those domains are required for phage adsorption and entry.
+This part is sequence coding for the D1 and D2 of the protein 3 of CTXΦ, a filamentous phage that target V.Cholerae. Those domains are required for phage adsorption and entry.
+This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
 <!-- Add more about the biology of this part here