Difference between revisions of "Part:BBa K1033916:Experience"
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<b> Charaterization of pH stability of amajLime </b> | <b> Charaterization of pH stability of amajLime </b> | ||
| − | <p>We grew C41 bacteria with parts | + | <p>We grew C41 bacteria with parts BBa_K1033916 with constituitive promoter: J23100, in 2XYT for 24 hours. Purifying the amajLime by Ion Exchange Chromatography and Hydrophobic Interaction Chromatography, we measured the fluoresece of purified amajLime, which is diluted to 10µg/100µl (total 200µl) in triplicates, in different buffers (ranges from pH2 to pH12). The result shows that the stability drops dramatically in condition below 6 and attains maxima fluorescence at pH 7</p> |
Fig.2 Vary pH attributed to different fluorescent intensity of RFP. | Fig.2 Vary pH attributed to different fluorescent intensity of RFP. | ||
<table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%"> | <table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%"> | ||
Revision as of 14:41, 26 August 2017
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Applications of BBa_K1033916
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UNIQe8c1f0427eecb8d6-partinfo-00000000-QINU UNIQe8c1f0427eecb8d6-partinfo-00000001-QINU
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Hong Kong-CUHK iGEM 2017 |
Fluorescent properties of amajLime Instead of chromorptein, we proposed that amajLime is a fluorescent protein with the property of showing pigmentation, like BBa_E1010. We charaterised spectral properties of amajLime and found its max excitation and emission wavelength at 445 nm nd 485 nm respectively. Fig.1 Emission and Excitation spectra
We grew C41 bacteria with parts BBa_K1033916 with constituitive promoter: J23100, in 2XYT for 24 hours. Purifying the amajLime by Ion Exchange Chromatography and Hydrophobic Interaction Chromatography, we measured the fluoresece of purified amajLime, which is diluted to 10µg/100µl (total 200µl) in triplicates, in different buffers (ranges from pH2 to pH12). The result shows that the stability drops dramatically in condition below 6 and attains maxima fluorescence at pH 7 Fig.2 Vary pH attributed to different fluorescent intensity of RFP.
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