Difference between revisions of "Part:BBa K2423002:Design"

 
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===References===
 
===References===
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1. Moraga AR, Nohales PF, Pérez JAF, Gómez-Gómez L. Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas. Planta. 2004 Oct 1;219(6):955–66.
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2. Crocus sativus glucosyltransferase 2 (GLT2) mRNA, complete cds. 2003 Nov 1; Available from: http://www.ncbi.nlm.nih.gov/nuccore/AY262037.1

Revision as of 12:25, 27 October 2017


UGTCs2 under the control of a constitutive promoter (BBa_J23106)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 593
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 766
    Illegal AgeI site found at 1121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made a complete device including promoter and RBS. We also included a 6x his tag for easy purification of the expressed protein.


Source

Crocus sativus

References

1. Moraga AR, Nohales PF, Pérez JAF, Gómez-Gómez L. Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas. Planta. 2004 Oct 1;219(6):955–66.

2. Crocus sativus glucosyltransferase 2 (GLT2) mRNA, complete cds. 2003 Nov 1; Available from: http://www.ncbi.nlm.nih.gov/nuccore/AY262037.1