Difference between revisions of "Part:BBa K2333007"
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This part is a superfolder GFP similar to Bba_1746916, but has four codon modifications at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A. It has a quick maturation time (~6 min) compared to wild type GFP (40 minutes). See BNID 110546. This part excites in the standard GFP range, and has maximal excitation emission 485/520nm. | This part is a superfolder GFP similar to Bba_1746916, but has four codon modifications at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A. It has a quick maturation time (~6 min) compared to wild type GFP (40 minutes). See BNID 110546. This part excites in the standard GFP range, and has maximal excitation emission 485/520nm. | ||
| − | This part was designed to be tagged with a protein degradation tag (see Bba_K2333001-K2333006), and as such has had | + | This part was designed to be tagged with a protein degradation tag (see Bba_K2333001-K2333006), and as such has had its stop codons removed. See <partinfo>Bba_K2066509</partinfo> for this part with both stop codons. |
Latest revision as of 18:26, 29 October 2017
Super Folder GFP (sfGFP) stop codons removed
This part is a superfolder GFP similar to Bba_1746916, but has four codon modifications at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A. It has a quick maturation time (~6 min) compared to wild type GFP (40 minutes). See BNID 110546. This part excites in the standard GFP range, and has maximal excitation emission 485/520nm.
This part was designed to be tagged with a protein degradation tag (see Bba_K2333001-K2333006), and as such has had its stop codons removed. See BBa_K2066509 for this part with both stop codons.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13