Difference between revisions of "Part:BBa K2333007"
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2333007 short</partinfo>
 
<partinfo>BBa_K2333007 short</partinfo>
 +
 
This part is a superfolder GFP similar to Bba_1746916, but has four codon modifications at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A. It has a quick maturation time (~6 min) compared to wild type GFP (40 minutes). See BNID 110546. This part excites in the standard GFP range, and has maximal excitation emission 485/520nm.
 
This part is a superfolder GFP similar to Bba_1746916, but has four codon modifications at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A. It has a quick maturation time (~6 min) compared to wild type GFP (40 minutes). See BNID 110546. This part excites in the standard GFP range, and has maximal excitation emission 485/520nm.
  

Revision as of 18:03, 29 June 2017


Super Folder GFP (sfGFP) stop codons removed

This part is a superfolder GFP similar to Bba_1746916, but has four codon modifications at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A. It has a quick maturation time (~6 min) compared to wild type GFP (40 minutes). See BNID 110546. This part excites in the standard GFP range, and has maximal excitation emission 485/520nm.

This part was designed to be tagged with a protein degradation tag (see Bba_K2333001-K2333006), and as such has had it's stop codons removed. See Bba_K2066509 for this part with both stop codons.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13