Difference between revisions of "Part:BBa K2333005"

Revision as of 23:03, 14 June 2017

mf-Lon Protein Degradation Tag E (medium-weak)

This protein degradation tag (pdt) is degraded by the Lon protease from Mycoplasma florum (mf-Lon). LINK TO mf-Lon goes here The Lon protease efficiently recognize and degrade C-terminal ssrA-tagged proteins, functioning in a similar way as ClpXP protease in most gram-negative bacteria. This Lon protease system is orthogonal to E. coli’s endogenous protein degradation machinery. This tag is part of a series of 6 tags (labeled #3 and #3a-#3e). pdt#3 is used as the parental tag to create #3a-#3e by changing the pdt residues 13–15 for mutagenesis because this region is essential for Lon-mediated degradation. Of this series of tags, this tag is the second weakest, and has the second slowest degradation rate. BBa_K2333001-Bba_K2333006 comprise this tag series. See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design via mutagenesis, and for their characterization of degradation rates.

Note: pdt #3d varies from pdt #3 in that residues 13-15 change from “PTF” to “APN”.

Sequence and Features