Difference between revisions of "Part:BBa K2333001"

Revision as of 00:15, 4 October 2017

mf-Lon Protein Degradation Tag A (strong)

This protein degradation tag (pdt) is degraded by the AAA+ Lon protease from Mesoplasma florum (mf-Lon) LINK TO mf-Lon goes here . While mf-Lon is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli tags (commonly known as ssRA tags), and vis versa.

This tag is part of a series of 6 tags (labeled #3 and #3a-#3e). Of this series of tags, this tag is the strongest (highest degradation rate). BBa_K2333001-Bba_K2333006 comprise this tag series.

See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design of these tags via mutagenesis, and for their characterization of degradation rates.

Note: Each pdt in the series BBa_K2333001-Bba_K2333006 (pdt#3-pdt#3e) is identical except for mutations on residues 13-15, which is the region of mf-Lon recognition.

Describe various degradation rates with parts here.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2333001 short</partinfo>
-This protein degradation tag (pdt) is degraded by the Lon protease from Mycoplasma florum (mf-Lon) <b> LINK TO mf-Lon goes here </b>. This Lon protease system is orthogonal to E. coli endogenous proteases. This tag is part of a series of 6 tags (labeled #3 and #3a-#3e). Of this series of tags, this tag is the strongest, and thus has the highest degradation rate. BBa_K2333001-Bba_K2333006 comprise this tag series. See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design via mutagenesis, and for their characterization of degradation rates.
+This protein degradation tag (pdt) is degraded by the AAA+ Lon protease from Mesoplasma florum  (mf-Lon) <b> LINK TO mf-Lon goes here </b>. While mf-Lon is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli tags (commonly known as ssRA tags), and vis versa.
+This tag is part of a series of 6 tags (labeled #3 and #3a-#3e). Of this series of tags, this tag is the strongest (highest degradation rate). BBa_K2333001-Bba_K2333006 comprise this tag series.
+See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design of these tags via mutagenesis, and for their characterization of degradation rates.
 Note: Each pdt in the series BBa_K2333001-Bba_K2333006 (pdt#3-pdt#3e) is identical except for mutations on residues 13-15, which is the region of mf-Lon recognition.
+<b> Describe various degradation rates with parts here. </b>
 <!-- Add more about the biology of this part here