Difference between revisions of "Part:BBa J100316"
 
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<partinfo>BBa_J100316 short</partinfo>
 
<partinfo>BBa_J100316 short</partinfo>
  
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J100316 is part J100284 with a GFP reporter after geneIII. This plasmid is used in the negative selection phase of PACE to allow for survival of the desired phage with the addition of aTc. The mutagenesis cassette was removed to prevent a high rate of mutagenesis in the negative selection phase.  
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J100316 is part J100284 with a GFP reporter after geneIII. J100316 is used in the negative selection phase of PACE to allow for survival of the desired phage but it requires the addition of aTc to induce gene III. The mutagenesis cassette was removed to prevent a high rate of mutagenesis in the negative selection phase.  
  
 
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Latest revision as of 13:09, 8 May 2017


J100284 with GFP reporter after geneIII

J100316 is part J100284 with a GFP reporter after geneIII. J100316 is used in the negative selection phase of PACE to allow for survival of the desired phage but it requires the addition of aTc to induce gene III. The mutagenesis cassette was removed to prevent a high rate of mutagenesis in the negative selection phase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3678
    Illegal PstI site found at 1525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1525
    Illegal NotI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1407
    Illegal BamHI site found at 2194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3678
    Illegal PstI site found at 1525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3678
    Illegal PstI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1059