Difference between revisions of "Part:BBa J100204"
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The OmpR-activated ompC promoter (R0082) is only present in part. The new insert will contain the 5' end of this promoter, allowing transcription. | The OmpR-activated ompC promoter (R0082) is only present in part. The new insert will contain the 5' end of this promoter, allowing transcription. | ||
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Revision as of 19:13, 12 July 2017
actClone Red
We designed actClone Red to test the power of activators. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and a DNA segment that binds to an activator to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.
The OmpR-activated ompC promoter (R0082) is only present in part. The new insert will contain the 5' end of this promoter, allowing transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 1739 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 1739 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 1739 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 1739
Illegal AgeI site found at 1612
Illegal AgeI site found at 1724 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 928
Illegal BsaI.rc site found at 817
Illegal SapI site found at 708