Difference between revisions of "Part:BBa M50055:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
We added an eGFP sequence downstream of our atrazine riboswitch so the the riboswitch would control translation of the eGFP.
+
We added an eGFP sequence downstream of our atrazine riboswitch (BBa_M50048) so that the riboswitch would control translation of the eGFP.
  
 
===Source===
 
===Source===

Revision as of 08:00, 12 December 2016


Atrazine biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 760


Design Notes

We added an eGFP sequence downstream of our atrazine riboswitch (BBa_M50048) so that the riboswitch would control translation of the eGFP.

Source

Riboswitch components: BBa_J89000

Atrazine aptamer: In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine, IJMS International Journal of Molecular Sciences

eGFP sequence: BBa_K598009

References

https://parts.igem.org/Part:BBa_K598009

https://parts.igem.org/Part:BBa_J89000

Williams, R., Crihfield, C., Gattu, S., Holland, L., & Sooter, L. (2014). In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. IJMS International Journal of Molecular Sciences, 15(8), 14332-14347. doi:10.3390/ijms150814332