Difference between revisions of "Part:BBa M50039:Design"
Abbiemcnulty (Talk | contribs) (→Source) |
Abbiemcnulty (Talk | contribs) (→References) |
||
Line 13: | Line 13: | ||
===References=== | ===References=== | ||
+ | “cusR response regulator in two-component regulatory system with cusS [Escherichia coli str. K-12 substr. MG1655”. (2016, Sep. 10). NCBI Reference Sequences.<BR><BR>Zahid N, Zulfiqar S, Shakoori AR. (2012, March). Functional analysis of cus operon promoter of Klebsiella pneumoniae using E. coli lacZ assay. Gene, 495(1):81-88. <BR><BR>Loftin IR, et al. (2005, July). A novel copper-binding fold for the periplasmic copper resistance protein cusF. Biochemistry, 44(31):10533-10540. |
Revision as of 23:50, 11 December 2016
E. coli positive feedback cusR producer (short promoter)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 53
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 53
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 53
Illegal AgeI site found at 243 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed a genetic construct where the first half consisted of the cusR inducible promoter, a strong ribosome binding site (RBS) listed in the Parts Registry (BBa_B0035) and thecusR gene sourced from NCBI.
Source
References
“cusR response regulator in two-component regulatory system with cusS [Escherichia coli str. K-12 substr. MG1655”. (2016, Sep. 10). NCBI Reference Sequences.
Zahid N, Zulfiqar S, Shakoori AR. (2012, March). Functional analysis of cus operon promoter of Klebsiella pneumoniae using E. coli lacZ assay. Gene, 495(1):81-88.
Loftin IR, et al. (2005, July). A novel copper-binding fold for the periplasmic copper resistance protein cusF. Biochemistry, 44(31):10533-10540.