Difference between revisions of "Part:BBa M50036"
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<partinfo>BBa_M50036 short</partinfo> | <partinfo>BBa_M50036 short</partinfo> | ||
| − | This sequence encodes for six amino acids that constitute the fusion protein linker for our Stanford BioE44 DNA construct. It is optimized for use in E. coli, and is designed to maintain linearity of the sequence and prevent protein folding. Our design relied on proper orientation of two | + | This sequence encodes for six amino acids that constitute the fusion protein linker for our Stanford BioE44 DNA construct. It is optimized for use in E. coli, and is designed to maintain linearity of the sequence and prevent protein folding. Our design relied on proper orientation of two glucose binding protein domains linked on each end to a flurophore. Thus the linkers were included to ensure that glucose-binding caused the conformational change characterized with FRET instead of inherently folded protein structure. |
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Latest revision as of 22:57, 11 December 2016
6 Amino Acid Fusion Protein Linker
This sequence encodes for six amino acids that constitute the fusion protein linker for our Stanford BioE44 DNA construct. It is optimized for use in E. coli, and is designed to maintain linearity of the sequence and prevent protein folding. Our design relied on proper orientation of two glucose binding protein domains linked on each end to a flurophore. Thus the linkers were included to ensure that glucose-binding caused the conformational change characterized with FRET instead of inherently folded protein structure.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]