Difference between revisions of "Part:BBa M50050:Experience"
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==Data Analysis and Conclusions== | ==Data Analysis and Conclusions== | ||
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+ | All figures show comparisons between the LuxS transfected bacteria, a wild-type control strain, and a similar IPTG-inducible E. coli strain with a T5 promoter (Pink). | ||
====Western Blot==== | ====Western Blot==== | ||
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Based on the data collected from our overnight cell density tests, we can conclude that the over-expression of LuxS increases cell growth during the exponential growth phase, but does not result in any vastly different final cell density concentrations. | Based on the data collected from our overnight cell density tests, we can conclude that the over-expression of LuxS increases cell growth during the exponential growth phase, but does not result in any vastly different final cell density concentrations. | ||
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+ | ==Experimental Procedures== | ||
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+ | ===Western Blot=== | ||
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+ | ===Cell Density Tests=== | ||
===User Reviews=== | ===User Reviews=== |
Revision as of 22:52, 11 December 2016
General Information
Applications of BBa_M50050
- Standardized growth control for bacteria species that have pathways for sensing and responding to Autoinducer-2.
Stanford Location
Plasmid Name: LuxS Synthase Plasmid
DNA 2.0 Gene #: pD441-CH
Organism: E. coli
Device Type: Actuator
Glycerol Stock Barcode: 0133027189
Box Label: BIOE44 F16
Data
Data Analysis and Conclusions
All figures show comparisons between the LuxS transfected bacteria, a wild-type control strain, and a similar IPTG-inducible E. coli strain with a T5 promoter (Pink).
Western Blot
Based on our analysis of our Western blot, we concluded that we were successful in transfecting our E. coli strain with the correct plasmid and gene sequence. For BBa_M50050, we looked at well sections A and B (BBa_M50050 transfected E. coli at 0 μM and 1000 μM in-solution concentration respectively) compared to the wild-type controls (C and F). What we see is that there are bands of proteins located at the 40 kilodaltons (the anticipated length) in sections A and B, but not in the control sections C and F.
Cell Density Tests
Based on the data collected from our overnight cell density tests, we can conclude that the over-expression of LuxS increases cell growth during the exponential growth phase, but does not result in any vastly different final cell density concentrations.
Experimental Procedures
Western Blot
Cell Density Tests
User Reviews
UNIQe5b58ffe4cdeddb6-partinfo-00000000-QINU UNIQe5b58ffe4cdeddb6-partinfo-00000001-QINU