Difference between revisions of "Part:BBa M50009"
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mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in fluorescent resonance energy transfer (FRET) analysis as an acceptor fluorophore when paired with another compatible fluorescent protein, like a GFP. Emission and absorption spectra must be analyzed for each fluorophore member in the FRET pair to ensure proper overlap and optimal FRET efficiency. | mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in fluorescent resonance energy transfer (FRET) analysis as an acceptor fluorophore when paired with another compatible fluorescent protein, like a GFP. Emission and absorption spectra must be analyzed for each fluorophore member in the FRET pair to ensure proper overlap and optimal FRET efficiency. | ||
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| + | Team Bielefeld-CeBiTec improved this part. The improved version BBa_KK3900021 can be found under the following link: https://parts.igem.org/wiki/index.php?title=Part:BBa_KK3900021 | ||
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Revision as of 06:25, 20 October 2021
mRuby3 fluorescent protein
mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in fluorescent resonance energy transfer (FRET) analysis as an acceptor fluorophore when paired with another compatible fluorescent protein, like a GFP. Emission and absorption spectra must be analyzed for each fluorophore member in the FRET pair to ensure proper overlap and optimal FRET efficiency.
Team Bielefeld-CeBiTec improved this part. The improved version BBa_KK3900021 can be found under the following link: https://parts.igem.org/wiki/index.php?title=Part:BBa_KK3900021
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 16